diff --git a/call/rtp_payload_params_unittest.cc b/call/rtp_payload_params_unittest.cc
index d3bdf5615e..90b08a27e7 100644
--- a/call/rtp_payload_params_unittest.cc
+++ b/call/rtp_payload_params_unittest.cc
@@ -397,7 +397,6 @@ class RtpPayloadParamsVp8ToGenericTest : public ::testing::Test {
         params_.GetRtpVideoHeader(encoded_image, &codec_info, shared_frame_id);
 
     ASSERT_TRUE(header.generic);
-    EXPECT_TRUE(header.generic->higher_spatial_layers.empty());
     EXPECT_EQ(header.generic->spatial_index, 0);
 
     EXPECT_EQ(header.generic->frame_id, shared_frame_id);
@@ -494,7 +493,6 @@ class RtpPayloadParamsH264ToGenericTest : public ::testing::Test {
         params_.GetRtpVideoHeader(encoded_image, &codec_info, shared_frame_id);
 
     ASSERT_TRUE(header.generic);
-    EXPECT_TRUE(header.generic->higher_spatial_layers.empty());
     EXPECT_EQ(header.generic->spatial_index, 0);
 
     EXPECT_EQ(header.generic->frame_id, shared_frame_id);
diff --git a/modules/rtp_rtcp/source/rtp_sender_video.cc b/modules/rtp_rtcp/source/rtp_sender_video.cc
index d1822f2997..eafcdf084e 100644
--- a/modules/rtp_rtcp/source/rtp_sender_video.cc
+++ b/modules/rtp_rtcp/source/rtp_sender_video.cc
@@ -121,11 +121,6 @@ void AddRtpHeaderExtensions(const RTPVideoHeader& video_header,
       }
 
       uint8_t spatial_bimask = 1 << video_header.generic->spatial_index;
-      for (int layer : video_header.generic->higher_spatial_layers) {
-        RTC_DCHECK_GT(layer, video_header.generic->spatial_index);
-        RTC_DCHECK_LT(layer, 8);
-        spatial_bimask |= 1 << layer;
-      }
       generic_descriptor.SetSpatialLayersBitmask(spatial_bimask);
 
       generic_descriptor.SetTemporalLayer(video_header.generic->temporal_index);
diff --git a/modules/rtp_rtcp/source/rtp_sender_video_unittest.cc b/modules/rtp_rtcp/source/rtp_sender_video_unittest.cc
index faf0decf98..1663ad5ea7 100644
--- a/modules/rtp_rtcp/source/rtp_sender_video_unittest.cc
+++ b/modules/rtp_rtcp/source/rtp_sender_video_unittest.cc
@@ -531,7 +531,6 @@ void RtpSenderVideoTest::PopulateGenericFrameDescriptor(int version) {
   generic.frame_id = kFrameId;
   generic.temporal_index = 3;
   generic.spatial_index = 2;
-  generic.higher_spatial_layers.push_back(4);
   generic.dependencies.push_back(kFrameId - 1);
   generic.dependencies.push_back(kFrameId - 500);
   hdr.frame_type = VideoFrameType::kVideoFrameDelta;
@@ -552,8 +551,7 @@ void RtpSenderVideoTest::PopulateGenericFrameDescriptor(int version) {
   EXPECT_EQ(static_cast<uint16_t>(generic.frame_id), descriptor_wire.FrameId());
   EXPECT_EQ(generic.temporal_index, descriptor_wire.TemporalLayer());
   EXPECT_THAT(descriptor_wire.FrameDependenciesDiffs(), ElementsAre(1, 500));
-  uint8_t spatial_bitmask = 0x14;
-  EXPECT_EQ(spatial_bitmask, descriptor_wire.SpatialLayersBitmask());
+  EXPECT_EQ(descriptor_wire.SpatialLayersBitmask(), 0b0000'0100);
 }
 
 TEST_P(RtpSenderVideoTest, PopulateGenericFrameDescriptor00) {
diff --git a/modules/rtp_rtcp/source/rtp_video_header.h b/modules/rtp_rtcp/source/rtp_video_header.h
index 78f8c4b2d1..9af2c094b6 100644
--- a/modules/rtp_rtcp/source/rtp_video_header.h
+++ b/modules/rtp_rtcp/source/rtp_video_header.h
@@ -43,7 +43,6 @@ struct RTPVideoHeader {
     int spatial_index = 0;
     int temporal_index = 0;
     absl::InlinedVector<int64_t, 5> dependencies;
-    absl::InlinedVector<int, 5> higher_spatial_layers;
     bool discardable = false;
   };