diff --git a/rtc_tools/BUILD.gn b/rtc_tools/BUILD.gn
index f6b03daed5..e707bfca16 100644
--- a/rtc_tools/BUILD.gn
+++ b/rtc_tools/BUILD.gn
@@ -69,7 +69,6 @@ rtc_static_library("y4m_file_reader") {
     "../api/video:video_frame",
     "../api/video:video_frame_i420",
     "../rtc_base:rtc_base_approved",
-    "../rtc_base:sequenced_task_checker",
     "//third_party/abseil-cpp/absl/types:optional",
   ]
 }
diff --git a/rtc_tools/y4m_file_reader.cc b/rtc_tools/y4m_file_reader.cc
index e2bc399fa8..ea8a741c65 100644
--- a/rtc_tools/y4m_file_reader.cc
+++ b/rtc_tools/y4m_file_reader.cc
@@ -20,7 +20,6 @@
 #include "api/video/i420_buffer.h"
 #include "rtc_base/logging.h"
 #include "rtc_base/refcountedobject.h"
-#include "rtc_base/sequenced_task_checker.h"
 #include "rtc_base/string_to_number.h"
 #include "rtc_base/stringencode.h"
 #include "rtc_base/stringutils.h"
@@ -184,7 +183,6 @@ size_t Y4mFile::number_of_frames() const {
 
 rtc::scoped_refptr<I420BufferInterface> Y4mFile::GetFrame(
     size_t frame_index) const {
-  RTC_DCHECK_CALLED_SEQUENTIALLY(&thread_checker_);
   RTC_CHECK_LT(frame_index, frame_positions_.size());
 
   fsetpos(file_, &frame_positions_[frame_index]);
diff --git a/rtc_tools/y4m_file_reader.h b/rtc_tools/y4m_file_reader.h
index b11beef28c..cb86c9e26b 100644
--- a/rtc_tools/y4m_file_reader.h
+++ b/rtc_tools/y4m_file_reader.h
@@ -17,7 +17,6 @@
 
 #include "api/video/video_frame.h"
 #include "rtc_base/refcount.h"
-#include "rtc_base/sequenced_task_checker.h"
 
 namespace webrtc {
 namespace test {
@@ -89,7 +88,6 @@ class Y4mFile : public Video {
   const int height_;
   const float fps_;
   const std::vector<fpos_t> frame_positions_;
-  const rtc::SequencedTaskChecker thread_checker_;
   FILE* const file_;
 };